PRESENCE OF EFFLUX PUMP MEDIATED ANTIBIOTIC RESISTANCE IN GRAM NEGATIVE BACTERIA ISOLATED FROM PRIMARY SCHOOL PUPILS IN AGO-IWOYE, OGUN STATE, NIGERIA
DOI:
https://doi.org/10.46881/ajsn.v5i0.134Keywords:
Carbonyl Cyanide m-Chlorophenyl hydrazone (CCCP), Efflux Pump System, (EPS), Gram Negative Bacteria.Abstract
This study determined the prevalence of efflux pump mediated antibiotic resistance in Gram negative bacteria from primary school pupils in Ago-Iwoye, Ogun State, Nigeria due to the global concerns of such mechanism in antibiotic extrusion. The isolation, characterization and identification of the isolated organisms were performed using standard techniques while the susceptibility patterns of the organisms was carried out following the recommended procedure of NCCLS. The resistant isolates were screened for active efflux pump system in the presence of an efflux pump inhibitor (EPI) (CCCP) and interpreted following standard procedure. Results obtained displays the prevalence rate of the isolated organisms in the following increasing order respectively: Serratia marcescens (3.4%), Enterobacter aerogenes (5.1%), Proteus mirabilis (6.7%), Klebsiella pneumoniae (16.9%), Pseudomonas aeruginosa (18.5%) and Escherichia coli (49.4%) for male subject while that of female subject were Serratia marcescens(3.1%), Enterobacter aerogenes(4.6%), Proteus mirabilis(7.2%), Klebsiella pneumoniae (17.0%), Pseudomonas aeruginosa (18.6%) and Escherichia coli (49.4%). The frequency of the isolated organisms differs according to gender but the variation was found not to be statistically significant (P>0.05). Pseudomonas aeruginosa, Proteus mirabilis and Escherichia coli demonstrated 100% efflux mediated resistance to all the tested antibiotics. In conclusion, Gram negative bacteria isolated in this study engineered their resistance mostly through efflux pump system.References
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