PHYLOGENETIC GROUP OF ESCHERICHIA COLI IN URINE OF CHILDREN ATTENDING A MATERNAL AND CHILD CENTRE, LAGOS
DOI:
https://doi.org/10.46881/ajsn.v7i0.159Keywords:
Urinary Tract Infection (UTI), Molecular characterization, Escherichia coli, ChildrenAbstract
Urinary tract infection (UTI) is one of the most common bacterial infections causing significant morbidity in children. This study determines the phylogenetic group of Escherichia coli isolated from the urine of children attending Maternal and Child Centre, Amuwo-Odofin, Lagos State. Clean voided midstream urine sample collected from 215 children aged five years and below . The urine samples were culture on Mac Conkey and blood agar, bacterial 5counts greater than or equal to 1 x 10CFU/ml were regarded as positive for UTI. Identification of isolates and antibiotic susceptibility test was performed using standard methods. Polymerase Chain Reaction was used to classify the isolated E. coli into A, B1, B2 and D phylogenetic groups using presence or absence of ChuA, Yja A and TspE4C2. The prevalence of UTI was 51.2% with preponderance in male 62.7% aged 24 – 35months 75.0%. Staphylococcus aureus 19.1% followed by Pseudomonas aeruginosa 12.7% and Escherichia coli10.0% were common bacterial isolates. The isolates were highly resistant to Augmentin (71.8%) and Ampicillin 91.8%; the Escherichia coli were resistant to Augmentin 54.5% and Ampicillin 100%. The E. coli were classed into B1,A, and D phylogenetic groups with percentages of 54.5%27.3% and 18.2% respectively. , The prevalence of bacteria UTI among children in this study was very high, the isolates were highly resistant to the antibiotics tested, the E.coli belong to phylogenetic groups A, B1 and D and all were resistant to Ampicillin.References
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