Screening of Different Crude Extracts of the Leaves of Momordica Charantia Linn. and Blighia Sapida Koenig for Alpha-amylase Inhibitory Potential
DOI:
https://doi.org/10.46881/ajsn.v2i1.32Keywords:
Momordica charantia, Blighia sapida, diabetes, alpha-amylase inhibition, hyperglycaemiaAbstract
Combating the scourge of diabetes which is a disease caused by a group of metabolic disorders of protein and carbohydrate digestion is a global issue. There is a general increase in the incidences of Type-2 diabetes worldwide with an attendant increase in mortality. Different groups of synthetic drugs are used to manage the condition including alpha-glucosidase inhibitors because decreasing postprandial hyperglycaemia is a major factor in managing Type-2 diabetes. However, they all have undesirable side effects resulting in the need for an alternative mode of treatment. Plants generally have been a huge source of effective therapeutic agents for various ailments with little or no side effects. Traditionally, Momordica charantia and Blighia sapida are plants used to treat diabetes with some proven scientific evidence. We set out to investigate the crude extracts of the leaves of both plants for probable alpha-amylase inhibitory potential. The leaves of both plants were macerated successively in hexane, ethyl-acetate and methanol. The resulting crude extracts were subjected to the alpha-amylase assay at a concentration range of 0.1 mg/mL-1.0 mg/mL. At 1.0 mg/mL, the ethyl acetate extract of Blighia sapida gave the highest inhibitory potential of 66.58% while Momordica charantia gave 45.28% which were significantly different from control at p <0.05. The hexane extracts of both (B. sapida and M. charantia) gave 28.98% and 22.28% respectively while their methanol extracts gave 23.70% and 18.75 mg/mL. This study shows that the ethyl acetate extracts of both plants possess moderate alpha-amylase inhibitory potential.
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