Microbial Population, Dehydrogenase and Urease Activities in Soils Polluted with Spent Engine and Diesel Oil
DOI:
https://doi.org/10.46881/ajsn.v4i0.84Keywords:
Bacteria, Fungi, Dehydrogenase, Urease, IncubationAbstract
Contamination of soil with diesel and lubricating oils disturbs the biological balance of soil. It alters the succession of microorganisms, which affects the activity of soil enzymes. Hence this study investigated the effect of spent engine and diesel oils on microbial population, dehydrogenase and urease activities in soils. Soil sample collected was incubated at 27oC for 7 days to stabilize. 5kg of the stabilized soil sample was separately treated with spent engine and diesel oil at 50ml, 100ml, 150ml and 200ml respectively. Each treatment was replicated 3 times on a complete randomized block design. Untreated soil samples were used as control. Soil samples were incubated in the dark at 40oC+ 0.5 for a period of 12 days. Nutrient agar was used for the enumeration of total heterotrophic bacteria while Potato Dextrose agar was used for fungal enumeration. Bacteria was incubated at 300C for 24-48 hours, while fungi was incubated at 25oC for 5 days. Soil dehydrogenase and urease activities were measured at two-day sampling intervals up to the 12thday. Bacterial and fungal counts declined significantly (P<0.05), initially after treatment with spent engine and diesel oils, followed by increased counts during incubation. Dehydrogenase and urease activities significantly increased (P<0.05) after treatment with spent engine and diesel oils. Microorganisms, dehydrogenase and urease activities can be used as indicators of soil pollution. Amplification of the enzyme production rates of microorganisms indigenous to petroleum polluted soils could help in bioremediation of the polluted soils.
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